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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL12A1 Polyclonal antibody (51113-1-AP)

    WB analysis using 51113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51113-1-AP (Collagen Type XII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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COL12A1 Polyclonal antibody

COL12A1 Polyclonal Antibody for WB, ELISA
Cat No. 51113-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COL12, Collagen Type XII, Collagen alpha-1(XII) chain, Collagen alpha 1(XII) chain, COL12A1L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, SKOV-3 cells, T-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51113-1-AP targets COL12A1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name collagen, type XII, alpha 1
Calculated molecular weight 333 kDa
Observed molecular weight333-460 kDa
GenBank accession numberNM_004370
Gene Symbol COL12A1
Gene ID (NCBI) 1303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99715
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL12A1 (Collagen alpha-1(XII) chain), also known as COL12A1L. It is predicted to be located in the ECM. Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix. The molecular weight of COL12A1 is 333 kDa. It is reported that the molecular weight of the protein is 268-460kDa (PMID: 31273343).

Protocols

Product Specific Protocols
WB protocol for COL12A1 antibody 51113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols