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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type XVI Polyclonal antibody

Collagen Type XVI Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 19802-1-AP

Synonyms

COL16A1, COL16, FP1572, Collagen alpha-1(XVI) chain, Collagen alpha 1(XVI) chain

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Collagen Type XVI Polyclonal antibody (19802-1-AP)

    WB analysis of human placenta using 19802-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19802-1-AP (Collagen Type XVI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Collagen Type XVI Polyclonal antibody (19802-1-AP)

    WB analysis of human placenta using 19802-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19802-1-AP (Collagen Type XVI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using Collagen Type XVI Polyclonal antibody (19802-1-AP)

    IF Staining of mouse skin using 19802-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Collagen Type XVI antibody (19802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IF-P detected inmouse skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19802-1-AP targets Collagen Type XVI in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name collagen, type XVI, alpha 1
Calculated molecular weight 158 kDa
Observed molecular weight 158 kDa
GenBank accession numberNM_001856
Gene symbol COL16A1
Gene ID (NCBI) 1307
RRIDAB_10638606
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL16A1, also named as FP1572, belongs to the fibril-associated collagens with interrupted helices (FACIT) family. COL16A1 is involved in mediating cell attachment and inducing integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology. The antibody is specific to COL16A1.

Protocols

Product Specific Protocols
WB protocol for Collagen Type XVI antibody 19802-1-APDownload protocol
IF protocol for Collagen Type XVI antibody 19802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

MicroRNA-152 and -181a participate in human dermal fibroblasts senescence acting on cell adhesion and remodeling of the extra-cellular matrix.

Authors - Mancini Mara M
View Article