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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COL17A1 Polyclonal antibody

COL17A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31190-1-AP

Synonyms

97 kDa LAD antigen, BP180, BPAG2, Bullous pemphigoid antigen 2, COL17A1, Collagen alpha 1(XVII) chain, collagen, type XVII, alpha 1, FLJ60881, KIAA0204, LABD97, LAD 1, Linear IgA disease antigen 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL17A1 Polyclonal antibody (31190-1-AP)

    WB analysis using 31190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat skin tissue using COL17A1 Polyclonal antibody (31190-1-AP)

    IHC staining of rat skin using 31190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using COL17A1 Polyclonal antibody (31190-1-AP)

    IHC staining of rat skin using 31190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using COL17A1 Polyclonal antibody (31190-1-AP)

    IF Staining of A431 using 31190-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using COL17A1 Polyclonal antibody (31190-1-AP)

    IF Staining of HaCaT using 31190-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inA431 cells, mouse skin tissue
Positive IHC detected inrat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HaCaT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31190-1-AP targets COL17A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COL17A1 fusion protein Ag34645 相同性解析による交差性が予測される生物種
Full Name collagen, type XVII, alpha 1
Observed molecular weight180 kDa
GenBank accession numberBC168368
Gene symbol COL17A1
Gene ID (NCBI) 1308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).

Protocols

Product Specific Protocols
WB protocol for COL17A1 antibody 31190-1-APDownload protocol
IHC protocol for COL17A1 antibody 31190-1-APDownload protocol
IF protocol for COL17A1 antibody 31190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols