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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

Collagen Type XXIII Polyclonal antibody

Collagen Type XXIII Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14337-1-AP

Synonyms

COL23A1, Collagen alpha 1(XXIII) chain, Collagen Type XXIII, collagen, type XXIII, alpha 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using Collagen Type XXIII Polyclonal antibody (14337-1-AP)

    WB analysis of mouse thymus using 14337-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using Collagen Type XXIII Polyclonal antibody (14337-1-AP)

    WB analysis of mouse thymus using 14337-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Collagen Type XXIII Polyclonal antibody (14337-1-AP)

    IHC staining of human colon using 14337-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Collagen Type XXIII Polyclonal antibody (14337-1-AP)

    IHC staining of human colon using 14337-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse thymus tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14337-1-AP targets Collagen Type XXIII in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type XXIII fusion protein Ag5640 相同性解析による交差性が予測される生物種
Full Name collagen, type XXIII, alpha 1
Calculated molecular weight 52 kDa
Observed molecular weight 68-75 kDa
GenBank accession numberBC042428
Gene symbol COL23A1
Gene ID (NCBI) 91522
RRIDAB_2276392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Collagen Type XXIII antibody 14337-1-APDownload protocol
IHC protocol for Collagen Type XXIII antibody 14337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

The Oncogenic Role of COL23A1 in Clear Cell Renal Cell Carcinoma.

Authors - Fujiang Xu
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Mikhail (Verified Customer) (06-05-2022)

I am satisfied with this product, it is suitable to use im my experiments

  • Applications: Flow Cytometry
  • Cell Tissue Type: Macrophages