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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL3A1 Monoclonal antibody (68320-1-Ig)

    WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using COL3A1 Monoclonal antibody (68320-1-Ig)

    WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human rectum tissue using COL3A1 Monoclonal antibody (68320-1-Ig)

    WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

    human rectum tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit large intestine tissue using COL3A1 Monoclonal antibody (68320-1-Ig)

    WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

    rabbit large intestine tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using COL3A1 Monoclonal antibody (68320-1-Ig)

    IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using COL3A1 Monoclonal antibody (68320-1-Ig)

    IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using COL3A1 Monoclonal antibody (68320-1-Ig)

    IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using COL3A1 Monoclonal antibody (68320-1-Ig)

    IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using COL3A1 Monoclonal antibody (68320-1-Ig)

    IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using COL3A1 Monoclonal antibody (68320-1-Ig)

    IF Staining of MCF-7 using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using COL3A1 antibody (68320-1-Ig, Clone: 3B1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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COL3A1 Monoclonal antibody, PBS Only

COL3A1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68320-1-PBS
Clone No.3B1F3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

COL3, 3B1F3, collagen, type III, alpha 1, Collagen Type III, Collagen alpha-1(III) chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68320-1-PBS targets COL3A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COL3A1 fusion protein Ag4229 相同性解析による交差性が予測される生物種
Full Name collagen, type III, alpha 1
Calculated molecular weight 1466 aa, 139 kDa
Observed molecular weight130 kDa
GenBank accession numberBC028178
Gene Symbol COL3A1
Gene ID (NCBI) 1281
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02461
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).