Collagen Type III (N-terminal) Recombinant antibody
Collagen Type III (N-terminal) Recombinant Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2O1
Cat no : 80009-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human colon tissue, human colon cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
80009-1-RR targets Collagen Type III (N-terminal) in IHC, IF-P, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Collagen Type III (N-terminal) fusion protein Ag18658 相同性解析による交差性が予測される生物種 |
Full Name | collagen, type III, alpha 1 |
Calculated molecular weight | 1466 aa, 139 kDa |
GenBank accession number | BC028178 |
Gene symbol | COL3A1 |
Gene ID (NCBI) | 1281 |
RRID | AB_2882939 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.
Protocols
Product Specific Protocols | |
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IHC protocol for Collagen Type III (N-terminal) antibody 80009-1-RR | Download protocol |
IF protocol for Collagen Type III (N-terminal) antibody 80009-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |