Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
18305-1-AP targets Collagen Type IV in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | collagen, type IV, alpha 3 (Goodpasture antigen) |
| Calculated molecular weight | 162 kDa |
| GenBank accession number | NM_000091 |
| Gene Symbol | COL4A3 |
| Gene ID (NCBI) | 1285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tumstatin is a cleavage fragment(C-term-245aa) corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms. Tumstatin indicated proapoptotic activity by selective inhibition of protein synthesis on endothelial cells. At normal physiologic levels, tumstatin, a cleavage product of the alpha3 chain of type IV collagen, is capable of suppressing pathologic angiogenesis and tumor growth, according to animal study findings published in the June issue of Cancer Cell.
Publications
| Species | Application | Title |
|---|---|---|
Food Funct Quercetin prevents the USP22-Snail1 signaling pathway to ameliorate diabetic tubulointerstitial fibrosis | ||
World J Diabetes Asiaticoside improves diabetic nephropathy by reducing inflammation, oxidative stress, and fibrosis: An in vitro and in vivo study |