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  • Featured Product
  • KD/KO Validated

COL4A3BP Polyclonal antibody, PBS Only

COL4A3BP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15191-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL4A3BP Polyclonal antibody (15191-1-AP)

    WB analysis using 15191-1-AP (same clone as 15191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    WB analysis of mouse pancreas using 15191-1-AP (same clone as 15191-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    WB analysis of mouse kidney using 15191-1-AP (same clone as 15191-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human pancreas tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    WB analysis of human pancreas using 15191-1-AP (same clone as 15191-1-PBS)

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using COL4A3BP Polyclonal antibody (15191-1-AP)

    WB analysis of HEK-293 using 15191-1-AP (same clone as 15191-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    WB analysis of rat brain using 15191-1-AP (same clone as 15191-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    IP experiment of mouse brain using 15191-1-AP (same clone as 15191-1-PBS)

    IP result of anti-COL4A3BP (IP:15191-1-AP, 4ug; Detection:15191-1-AP 1:300) with mouse brain tissue lysate 3680ug. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    IHC staining of human pancreas using 15191-1-AP (same clone as 15191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    IHC staining of human pancreas using 15191-1-AP (same clone as 15191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    IHC staining of human kidney using 15191-1-AP (same clone as 15191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using COL4A3BP Polyclonal antibody (15191-1-AP)

    IHC staining of human kidney using 15191-1-AP (same clone as 15191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15191-1-PBS targets COL4A3BP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COL4A3BP fusion protein Ag7277 相同性解析による交差性が予測される生物種
Full Name collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
Calculated molecular weight 71 kDa
Observed molecular weight 80-89 kDa
GenBank accession numberBC000102
Gene symbol COL4A3BP
Gene ID (NCBI) 10087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COL4A3BP(Collagen type IV alpha-3-binding protein) is also named as CERT, STARD11. CERT extracts ceramide from the ER and carries it to the Golgi apparatus in a non-vesicular manner and that a particularly efficient cycle of CERT movement for trafficking of ceramide may proceed at membrane contact sites between the ER and the Golgi apparatus(PMID:17314061). This protein has 3 isoforms produced by alternative splicing.