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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL5A3 Polyclonal antibody (30204-1-AP)

    WB analysis using 30204-1-AP (same clone as 30204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30204-1-AP (COL5A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30204-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using COL5A3 Polyclonal antibody (30204-1-AP)

    IF Staining of HUVEC using 30204-1-AP (same clone as 30204-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using COL5A3 antibody (30204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30204-1-PBS in a different storage buffer formulation.

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COL5A3 Polyclonal antibody, PBS Only

COL5A3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30204-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

COL5, Collagen Type V, Collagen alpha-3(V) chain, Collagen alpha 3(V) chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30204-1-PBS targets COL5A3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COL5A3 fusion protein Ag30705 相同性解析による交差性が予測される生物種
Full Name collagen, type V, alpha 3
Calculated molecular weight 172 kDa
Observed molecular weight145 kDa
GenBank accession numberNM_015719
Gene Symbol COL5A3
Gene ID (NCBI) 50509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25940
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COL5A3 (Collagen type V alpha 3) belongs to the fibrillar collagen family. Type V collagen (COLV) is a member of a group I collagen (fibrillar forming collagen) and has three varieties of α chains: α1 (COL5A1), α2 (COL5A2), and α3 (COL5A3). COLV is a minor connective tissue component of nearly ubiquitous distribution. It binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This antibody is specific to COL5A3.