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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using COL6A3 Polyclonal antibody (19798-1-AP)

    IHC staining of human cervical cancer using 19798-1-AP (same clone as 19798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19798-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using COL6A3 Polyclonal antibody (19798-1-AP)

    IHC staining of human lung cancer using 19798-1-AP (same clone as 19798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19798-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using COL6A3 Polyclonal antibody (19798-1-AP)

    IHC staining of human urothelial carcinoma using 19798-1-AP (same clone as 19798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 19798-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19798-1-PBS in a different storage buffer formulation.

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COL6A3 Polyclonal antibody, PBS Only

COL6A3 Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 19798-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

Collagen alpha 3(VI) chain, Collagen alpha-3(VI) chain, Collagen Type VI

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19798-1-PBS targets COL6A3 in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 3
Calculated molecular weight 344 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_004369
Gene Symbol COL6A3
Gene ID (NCBI) 1293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12111
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.