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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL6A6 Polyclonal antibody (22302-1-AP)

    WB analysis using 22302-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22302-1-AP (COL6A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using COL6A6 Polyclonal antibody (22302-1-AP)

    IHC staining of mouse lung using 22302-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using COL6A6 Polyclonal antibody (22302-1-AP)

    IHC staining of mouse heart using 22302-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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COL6A6 Polyclonal antibody

COL6A6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22302-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

COL6A6, Collagen alpha 6(VI) chain, collagen type VI alpha 6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

22302-1-AP targets COL6A6 in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples.

Tested Reactivity human, Mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name collagen type VI alpha 6
Calculated molecular weight 247 kDa
Observed molecular weight167 kDa, 250 kDa
GenBank accession numberNM_001102608
Gene Symbol COL6A6
Gene ID (NCBI) 131873
RRIDAB_3085695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NMZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collagen type VI alpha 6 chain (COL6A6) belongs to the collagen VI (COL6) family which acts as a vital role in the extracellular matrix. COL6A6 encodes a 2,262-amino acid protein that contains multiple von Willebrand factor domains and forms a component of the basal lamina of epithelial cells (PMID: 33747955). COL6A6 is a novel tumor suppressor gene in NSCLC and can serve as a potentially valuable prognostic biomarker and therapeutic target for NSCLC (PMID: 35116307).

Protocols

Product Specific Protocols
WB protocol for COL6A6 antibody 22302-1-APDownload protocol
IHC protocol for COL6A6 antibody 22302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Agric Food Chem

Combination of Structural Analysis and Proteomics Strategy Revealed the Mechanism of Ultrasound-Assisted Cold Plasma Regulating Shrimp Allergy

Authors - Hangyu Zhong
View Article