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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type IX Polyclonal antibody (12507-1-AP)

    WB analysis using 12507-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12507-1-AP (Collagen Type IX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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COL9A1 Polyclonal antibody

COL9A1 Polyclonal Antibody for WB, ELISA
Cat No. 12507-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COL9, Collagen alpha 1(IX) chain, Collagen alpha-1(IX) chain, Collagen Type IX, EDM6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12507-1-AP targets COL9A1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COL9A1 fusion protein Ag3189 相同性解析による交差性が予測される生物種
Full Name collagen, type IX, alpha 1
Calculated molecular weight 921 aa 92 kDa
Observed molecular weight64-67 kDa
GenBank accession numberBC015409
Gene Symbol COL9A1
Gene ID (NCBI) 1297
RRIDAB_3669142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20849
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL9A1 (collagen type IX alpha 1 chain), also known as MED. It is expected to be located in extracellular space, and the protein is enriched in the brain, choroid plexus and prostate. This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. The molecular weight of COL9A1 is 64 kDa, and it can recognize other isoforms.

Protocols

Product Specific Protocols
WB protocol for COL9A1 antibody 12507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols