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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COMMD1 Monoclonal antibody, PBS Only

COMMD1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B10E10

Cat no : 67016-1-PBS

Synonyms

C2orf5, COMMD1, MURR1, Protein Murr1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COMMD1 Monoclonal antibody (67016-1-Ig)

    WB analysis using 67016-1-Ig (same clone as 67016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67016-1-Ig (COMMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COMMD1 Monoclonal antibody (67016-1-Ig)

    IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COMMD1 Monoclonal antibody (67016-1-Ig)

    IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COMMD1 Monoclonal antibody (67016-1-Ig)

    IF Staining of HepG2 using 67016-1-Ig (same clone as 67016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COMMD1 antibody (67016-1-Ig, Clone: 3B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67016-1-PBS targets COMMD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMMD1 fusion protein Ag28596 相同性解析による交差性が予測される生物種
Full Name copper metabolism (Murr1) domain containing 1
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC022046
Gene symbol COMMD1
Gene ID (NCBI) 150684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.