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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COMMD3 Polyclonal antibody

COMMD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26240-1-AP

Synonyms

BUP, C10orf8, COMM domain containing 3, COMMD3, Protein Bup, Protein PIL

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using COMMD3 Polyclonal antibody (26240-1-AP)

    WB analysis of A549 using 26240-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26240-1-AP (COMMD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using COMMD3 Polyclonal antibody (26240-1-AP)

    WB analysis of mouse thymus using 26240-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 26240-1-AP (COMMD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thymus tissue using COMMD3 Polyclonal antibody (26240-1-AP)

    IHC staining of human thymus using 26240-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 26240-1-AP (COMMD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thymus tissue using COMMD3 Polyclonal antibody (26240-1-AP)

    IHC staining of human thymus using 26240-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 26240-1-AP (COMMD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, mouse thymus tissue
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 26240-1-AP is COMMD3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COMMD3 fusion protein Ag23968 相同性解析による交差性が予測される生物種
Full Name COMM domain containing 3
Observed molecular weight 22 kDa
GenBank accession numberBC022898
Gene symbol COMMD3
Gene ID (NCBI) 23412
RRIDAB_2880440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COMMD3 antibody 26240-1-APDownload protocol
IHC protocol for COMMD3 antibody 26240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
View Article
humanWB

Mol Metab

Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.

Authors - Liu Yang
View Article