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KD/KO Validated

COMMD5 Polyclonal antibody

Name: COMMD5 Polyclonal antibody
Brand: Proteintech
SKU: 10393-1-AP

COMMD5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10393-1-AP

Synonyms

Hypertension-related calcium-regulated gene protein, HT002, HCaRG, COMM domain-containing protein 5, COMM domain containing 5

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of mouse kidney using 10393-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10393-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10393-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10393-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10393-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 10393-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10393-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse stomach using 10393-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 10393-1-AP
IP result of anti-COMMD5 (IP:10393-1-AP, 4ug; Detection:10393-1-AP 1:500) with mouse heart tissue lysate 3200ug.
IF Staining of HeLa using 10393-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10393-1-AP (COMMD5 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10393-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, human heart tissue, human stomach tissue, mouse stomach tissue, mouse heart tissue, HEK-293 cells, MCF-7 cells, human placenta tissue
Positive IP detected in	mouse heart tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 4 publications below
IF	See 2 publications below
CoIP	See 2 publications below

Product Information

10393-1-AP targets COMMD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	COMMD5 fusion protein Ag0592 相同性解析による交差性が予測される生物種
Full Name	COMM domain containing 5
Calculated molecular weight	25 kDa
Observed molecular weight	25 kDa
GenBank accession number	BC003055
Gene symbol	COMMD5
Gene ID (NCBI)	28991
RRID	AB_2083555
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.

Protocols

Product Specific Protocols
WB protocol for COMMD5 antibody 10393-1-AP	Download protocol
IF protocol for COMMD5 antibody 10393-1-AP	Download protocol
IP protocol for COMMD5 antibody 10393-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	J Am Soc Nephrol HCaRG accelerates tubular repair after ischemic kidney injury. Authors - Hiroyuki Matsuda View Article
human	WB,IF,CoIP	Cell Rep COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking. Authors - Carole G Campion KD Validated View Article
human	IF	Elife Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes. Authors - Sai Srinivas Panapakkam Giridharan View Article
human	WB	Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. Authors - Liu Yang View Article
human	IHC	Oncol Rep PMID: 18949406 Authors - Chen Bai-Li BL View Article
human	IHC	Anticancer Res COMMD5 Inhibits Malignant Behavior of Renal Cancer Cells. Authors - Jin Ikeda View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COMMD5 Polyclonal antibody

COMMD5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of mouse kidney using 10393-1-AP

WB analysis of human placenta using 10393-1-AP

WB analysis of mouse heart using 10393-1-AP

WB analysis of HEK-293 using 10393-1-AP

WB analysis of human heart using 10393-1-AP

WB analysis of human stomach using 10393-1-AP

WB analysis of MCF-7 using 10393-1-AP

WB analysis of mouse stomach using 10393-1-AP

IP experiment of mouse heart using 10393-1-AP

IF Staining of HeLa using 10393-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

HCaRG accelerates tubular repair after ischemic kidney injury.

COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.

Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.

Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.

PMID: 18949406

COMMD5 Inhibits Malignant Behavior of Renal Cancer Cells.