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Validation Data Gallery

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  • Western Blot (WB) analysis of pig cartilage tissue using COMP Monoclonal antibody (66793-1-Ig)

    WB analysis of pig cartilage using 66793-1-Ig

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66793-1-Ig (COMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COMP Monoclonal antibody (66793-1-Ig)

    IF Staining of HepG2 using 66793-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using COMP Monoclonal antibody (66793-1-Ig)

    IF Staining of U2OS using 66793-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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COMP Monoclonal antibody

COMP Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66793-1-Ig
Clone No.1D7A6

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, ELISA

Synonyms

COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig cartilage tissue
Positive IF/ICC detected inHepG2 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66793-1-Ig targets COMP in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMP fusion protein Ag4575 相同性解析による交差性が予測される生物種
Full Name cartilage oligomeric matrix protein
Calculated molecular weight 757 aa, 83 kDa
Observed molecular weight 100-130 kDa
GenBank accession numberBC033676
Gene Symbol COMP
Gene ID (NCBI) 1311
RRIDAB_2882137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others. This antibody got 100-130 kDa maybe due to glycosylation.

Protocols

Product Specific Protocols
WB protocol for COMP antibody 66793-1-IgDownload protocol
IF protocol for COMP antibody 66793-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols