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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPA Recombinant antibody, PBS Only

COPA Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230361G2

Cat no : 83089-1-PBS

Synonyms

HEP COP, FLJ26320, Coatomer subunit alpha, Alpha COP, Alpha coat protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPA Recombinant antibody (83089-1-RR)

    WB analysis using 83089-1-RR (same clone as 83089-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83089-1-RR (COPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COPA Recombinant antibody (83089-1-RR)

    IHC staining of mouse liver using 83089-1-RR (same clone as 83089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83089-1-RR (COPA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COPA Recombinant antibody (83089-1-RR)

    IHC staining of mouse liver using 83089-1-RR (same clone as 83089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83089-1-RR (COPA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using COPA Recombinant antibody (83089-1-RR)

    IHC staining of human stomach using 83089-1-RR (same clone as 83089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 83089-1-RR (COPA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83089-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COPA Recombinant antibody (83089-1-RR)

    IF Staining of HeLa using 83089-1-RR (same clone as 83089-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using COPA antibody (83089-1-RR, Clone: 230361G2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83089-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using COPA Recombinant antibody (83089-1-RR)

    FC experiment of HeLa using 83089-1-RR (same clone as 83089-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human COPA (83089-1-RR, Clone:230361G2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83089-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83089-1-RR

    Affinity and Kinetic Characterization of 83089-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83089-1-RR against Human COPA were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83089-1-PBS targets COPA in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COPA fusion protein Ag34207 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit alpha
Calculated molecular weight138 kDa
Observed molecular weight150 kDa
GenBank accession numberNM_004371
Gene symbol COPA
Gene ID (NCBI) 1314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.