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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPB1 Polyclonal antibody

COPB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27469-1-AP

Synonyms

COPB, Coatomer subunit beta, Beta-COP, Beta-coat protein, Beta COP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPB1 Polyclonal antibody (27469-1-AP)

    WB analysis using 27469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27469-1-AP (COPB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using COPB1 Polyclonal antibody (27469-1-AP)

    IHC staining of human stomach using 27469-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27469-1-AP (COPB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27469-1-AP targets COPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPB1 fusion protein Ag26518 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit beta 1
Calculated molecular weight 107 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC037280
Gene symbol COPB1
Gene ID (NCBI) 1315
RRIDAB_2880880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPB1 antibody 27469-1-APDownload protocol
IHC protocol for COPB1 antibody 27469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols