• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPB2 Polyclonal antibody

COPB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14733-1-AP

Synonyms

p102, Coatomer subunit beta', Beta'-COP, Beta'-coat protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using COPB2 Polyclonal antibody (14733-1-AP)

    WB analysis using 14733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14733-1-AP (COPB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using COPB2 Polyclonal antibody (14733-1-AP)

    IHC staining of human stomach using 14733-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14733-1-AP (COPB2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using COPB2 Polyclonal antibody (14733-1-AP)

    IHC staining of human stomach cancer using 14733-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14733-1-AP (COPB2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using COPB2 Polyclonal antibody (14733-1-AP)

    IF Staining of PC-12 using 14733-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using COPB2 Polyclonal antibody (14733-1-AP)

    IF Staining of NIH/3T3 using 14733-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14733-1-AP targets COPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPB2 fusion protein Ag6498 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit beta 2 (beta prime)
Calculated molecular weight 102 kDa
Observed molecular weight102 kDa
GenBank accession numberBC000326
Gene symbol COPB2
Gene ID (NCBI) 9276
RRIDAB_3085441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPB2 antibody 14733-1-APDownload protocol
IHC protocol for COPB2 antibody 14733-1-APDownload protocol
IF protocol for COPB2 antibody 14733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols