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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPB2 Polyclonal antibody

COPB2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14733-1-AP

Synonyms

p102, Coatomer subunit beta', Beta'-COP, Beta'-coat protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPB2 Polyclonal antibody (14733-1-AP)

    WB analysis using 14733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14733-1-AP (COPB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using COPB2 Polyclonal antibody (14733-1-AP)

    IF Staining of PC-12 using 14733-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using COPB2 Polyclonal antibody (14733-1-AP)

    IF Staining of NIH/3T3 using 14733-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inPC-12 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14733-1-AP targets COPB2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPB2 fusion protein Ag6498 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit beta 2 (beta prime)
Calculated molecular weight 102 kDa
Observed molecular weight102 kDa
GenBank accession numberBC000326
Gene symbol COPB2
Gene ID (NCBI) 9276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPB2 antibody 14733-1-APDownload protocol
IF protocol for COPB2 antibody 14733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols