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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPG Polyclonal antibody

COPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12393-1-AP

Synonyms

Gamma-1-COP, Gamma-1-coat protein, COPG1, Coatomer subunit gamma-1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using COPG Polyclonal antibody (12393-1-AP)

    WB analysis using 12393-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using COPG Polyclonal antibody (12393-1-AP)

    WB analysis of A375 using 12393-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using COPG Polyclonal antibody (12393-1-AP)

    WB analysis of human colon using 12393-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using COPG Polyclonal antibody (12393-1-AP)

    WB analysis of human brain using 12393-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using COPG Polyclonal antibody (12393-1-AP)

    WB analysis of HeLa using 12393-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using COPG Polyclonal antibody (12393-1-AP)

    IP experiment of K-562 using 12393-1-AP

    IP result of anti-COPG (IP:12393-1-AP, 4ug; Detection:12393-1-AP 1:2000) with K-562 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using COPG Polyclonal antibody (12393-1-AP)

    IHC staining of mouse kidney using 12393-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12393-1-AP (COPG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using COPG Polyclonal antibody (12393-1-AP)

    IHC staining of mouse kidney using 12393-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12393-1-AP (COPG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COPG Polyclonal antibody (12393-1-AP)

    IHC staining of human breast cancer using 12393-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12393-1-AP (COPG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COPG Polyclonal antibody (12393-1-AP)

    IHC staining of human breast cancer using 12393-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COPG Polyclonal antibody (12393-1-AP)

    IHC staining of human breast cancer using 12393-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COPG Polyclonal antibody (12393-1-AP)

    IF Staining of HepG2 using 12393-1-AP

    Immunofluorescent analysis of HepG2 cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COPG Polyclonal antibody (12393-1-AP)

    IF Staining of HepG2 using 12393-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COPG antibody (12393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using COPG Polyclonal antibody (12393-1-AP)

    IF Staining of NIH/3T3 using 12393-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPG antibody (12393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COPG Polyclonal antibody (12393-1-AP)

    IF Staining of HeLa using 12393-1-AP

    Immunofluorescent analysis of Hela cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inK-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12393-1-AP targets COPG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPG fusion protein Ag3070 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit gamma
Calculated molecular weight 874 aa, 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC020498
Gene symbol COPG
Gene ID (NCBI) 22820
RRIDAB_2081435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPG antibody 12393-1-APDownload protocol
IHC protocol for COPG antibody 12393-1-APDownload protocol
IF protocol for COPG antibody 12393-1-APDownload protocol
IP protocol for COPG antibody 12393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Deficiency in coatomer complex I causes aberrant activation of STING signalling.

Authors - Annemarie Steiner
View Article
IF

J Exp Med

Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation.

Authors - Masahiko Nishitani-Isa
View Article
mouseWB

J Biol Chem

Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.

Authors - Xiaoling Li
View Article
mouseWB

Hum Mol Genet

Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.

Authors - Desiree M Baron
View Article
humanIP,IF,WB

Sci Adv

HPV is a cargo for the COPI sorting complex during virus entry

Authors - Mara C Harwood
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

RICHA (Verified Customer) (03-02-2022)

Great antibody for IF

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse tendon fibroblast
FH

RICHA (Verified Customer) (11-16-2020)

Excellent antibody

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse fibroblast
FH

RICHA (Verified Customer) (12-03-2019)

Excellent, no background

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse fibroblast