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  • Featured Product
  • KD/KO Validated

COPS3 Polyclonal antibody

COPS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30494-1-AP

Synonyms

JAB1-containing signalosome subunit 3, COP 9 signalosome complex subunit 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPS3 Polyclonal antibody (30494-1-AP)

    WB analysis using 30494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30494-1-AP (COPS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using COPS3 Polyclonal antibody (30494-1-AP)

    WB analysis of HeLa using 30494-1-AP

    WB result of COPS3 antibody (30494-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COPS3 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COPS3 Polyclonal antibody (30494-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30494-1-AP (COPS3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COPS3 Polyclonal antibody (30494-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30494-1-AP (COPS3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30494-1-AP targets COPS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPS3 fusion protein Ag32825 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
Calculated molecular weight 48 kDa
Observed molecular weight40 kDa
GenBank accession numberBC001891
Gene symbol COPS3
Gene ID (NCBI) 8533
RRIDAB_3086336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Protocols

Product Specific Protocols
WB protocol for COPS3 antibody 30494-1-APDownload protocol
IHC protocol for COPS3 antibody 30494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols