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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPS3 Recombinant antibody, PBS Only (Detector)

COPS3 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240918E3

Cat no : 83822-2-PBS

Synonyms

JAB1-containing signalosome subunit 3, COP 9 signalosome complex subunit 3, 240918E3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPS3 Recombinant antibody (83822-2-RR)

    WB analysis using 83822-2-RR (same clone as 83822-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83822-2-RR (COPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using COPS3 Recombinant antibody (83822-2-RR)

    IHC staining of mouse brain using 83822-2-RR (same clone as 83822-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83822-2-RR (COPS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using COPS3 Recombinant antibody (83822-2-RR)

    IF Staining of rat brain using 83822-2-RR (same clone as 83822-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using COPS3 Recombinant antibody (83822-2-RR)

    IF Staining of A431 using 83822-2-RR (same clone as 83822-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COPS3 Recombinant antibody (83822-2-RR)

    IF Staining of HeLa using 83822-2-RR (same clone as 83822-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00797-1

    Cytometric bead array standard curve of MP00797-1

    Cytometric bead array standard curve of MP00797-1, COPS3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83822-4-PBS. Detection antibody: 83822-2-PBS. Standard: Ag32825. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83822-2-RR

    Affinity and Kinetic Characterization of 83822-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83822-2-RR against Human COPS3 were performed. The affinity constant is 1.29 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83822-2-PBS targets COPS3 as part of a matched antibody pair:

MP00797-1: 83822-4-PBS capture and 83822-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COPS3 fusion protein Ag32825 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
Calculated molecular weight 48 kDa
Observed molecular weight46 kDa
GenBank accession numberBC001891
Gene symbol COPS3
Gene ID (NCBI) 8533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.