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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPS4 Polyclonal antibody

COPS4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10464-1-AP

Synonyms

COPS4, CSN4, SGN4, Signalosome subunit 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using COPS4 Polyclonal antibody (10464-1-AP)

    WB analysis of mouse brain using 10464-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using COPS4 Polyclonal antibody (10464-1-AP)

    WB analysis of PC-3 using 10464-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using COPS4 Polyclonal antibody (10464-1-AP)

    IP experiment of PC-3 using 10464-1-AP

    IP result of anti-COPS4 (IP:10464-1-AP, 4ug; Detection:10464-1-AP 1:300) with PC-3 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COPS4 Polyclonal antibody (10464-1-AP)

    IHC staining of human pancreas using 10464-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COPS4 Polyclonal antibody (10464-1-AP)

    IHC staining of human pancreas using 10464-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COPS4 Polyclonal antibody (10464-1-AP)

    IHC staining of human pancreas using 10464-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10464-1-AP (COPS4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COPS4 Polyclonal antibody (10464-1-AP)

    IHC staining of human pancreas using 10464-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10464-1-AP (COPS4 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10464-1-AP targets COPS4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPS4 fusion protein Ag0735 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis)
Calculated molecular weight 40; 46 kDa
Observed molecular weight 40 kDa, 46 kDa
GenBank accession numberBC004302
Gene symbol COPS4
Gene ID (NCBI) 51138
RRIDAB_2081630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an ess ential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. CSN4 gene encodes a component of CSN complex, COP9 signalosome complex subunit 4 (COPS4). The ubiquitin-proteasome system plays a major role in the rhythmic accumulation and turnover of molecular clock components. A recent study in Drosophila indicates that CSN lie in a common pathway leading to light-dependent degradation of clock protein Timeless (TIM).

Protocols

Product Specific Protocols
WB protocol for COPS4 antibody 10464-1-APDownload protocol
IHC protocol for COPS4 antibody 10464-1-APDownload protocol
IP protocol for COPS4 antibody 10464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuropsychiatr Dis Treat

COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein.

Authors - En Liang
View Article