Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
10464-1-AP targets COPS4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COPS4 fusion protein Ag0735 相同性解析による交差性が予測される生物種 |
| Full Name | COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis) |
| Calculated molecular weight | 40; 46 kDa |
| Observed molecular weight | 40 kDa, 46 kDa |
| GenBank accession number | BC004302 |
| Gene Symbol | COPS4 |
| Gene ID (NCBI) | 51138 |
| RRID | AB_2081630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BT78 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an ess ential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. CSN4 gene encodes a component of CSN complex, COP9 signalosome complex subunit 4 (COPS4). The ubiquitin-proteasome system plays a major role in the rhythmic accumulation and turnover of molecular clock components. A recent study in Drosophila indicates that CSN lie in a common pathway leading to light-dependent degradation of clock protein Timeless (TIM).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COPS4 antibody 10464-1-AP | Download protocol |
| IHC protocol for COPS4 antibody 10464-1-AP | Download protocol |
| IP protocol for COPS4 antibody 10464-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuropsychiatr Dis Treat COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. | ||
bioRxiv Crosslinking-mediated Interactome Analysis Identified PHD2-HIF1α Interaction Hotspots and the Role of PHD2 in Regulating Protein Neddylation |