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COPS6 Polyclonal antibody
COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig, monkey
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 10256-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse colon tissue, 3T3-L1 cells, pig lymph node tissue |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, COLO 320 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
Product Information
10256-1-AP targets COPS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.
Tested Reactivity | human, mouse, rat, pig, monkey |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | COPS6 fusion protein Ag0331 相同性解析による交差性が予測される生物種 |
Full Name | COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis) |
Calculated molecular weight | 327 aa, 36 kDa |
Observed molecular weight | 34 kDa |
GenBank accession number | BC002520 |
Gene symbol | COPS6 |
Gene ID (NCBI) | 10980 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.
Protocols
Product Specific Protocols | |
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WB protocol for COPS6 antibody 10256-1-AP | Download protocol |
IHC protocol for COPS6 antibody 10256-1-AP | Download protocol |
IF protocol for COPS6 antibody 10256-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Biol Sci CSN6 Promotes the Migration and Invasion of Cervical Cancer Cells by Inhibiting Autophagic Degradation of Cathepsin L.
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