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  • Featured Product
  • KD/KO Validated

COPS6 Polyclonal antibody

COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, monkey

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10256-1-AP

Synonyms

CSN6, Signalosome subunit 6, SGN6, MOV34 34KD, JAB1-containing signalosome subunit 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPS6 Polyclonal antibody (10256-1-AP)

    WB analysis using 10256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 3T3-L1 cells using COPS6 Polyclonal antibody (10256-1-AP)

    WB analysis of 3T3-L1 using 10256-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using COPS6 Polyclonal antibody (10256-1-AP)

    IHC staining of human colon using 10256-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using COPS6 Polyclonal antibody (10256-1-AP)

    IHC staining of human colon using 10256-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using COPS6 Polyclonal antibody (10256-1-AP)

    IF Staining of COLO 320 using 10256-1-AP

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COPS6 Polyclonal antibody (10256-1-AP)

    IF Staining of HeLa using 10256-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inmouse colon tissue, 3T3-L1 cells, pig lymph node tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10256-1-AP targets COPS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

Tested Reactivity human, mouse, rat, pig, monkey
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPS6 fusion protein Ag0331 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
Calculated molecular weight 327 aa, 36 kDa
Observed molecular weight34 kDa
GenBank accession numberBC002520
Gene symbol COPS6
Gene ID (NCBI) 10980
RRIDAB_2034908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.

Protocols

Product Specific Protocols
WB protocol for COPS6 antibody 10256-1-APDownload protocol
IHC protocol for COPS6 antibody 10256-1-APDownload protocol
IF protocol for COPS6 antibody 10256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

CSN6 Promotes the Migration and Invasion of Cervical Cancer Cells by Inhibiting Autophagic Degradation of Cathepsin L.

Authors - Zun Mao
  • KD Validated
View Article