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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPS7A Polyclonal antibody

COPS7A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16628-1-AP

Synonyms

COPS7A, CSN7A, DERP10, SGN7a, Signalosome subunit 7a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPS7A Polyclonal antibody (16628-1-AP)

    WB analysis using 16628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using COPS7A Polyclonal antibody (16628-1-AP)

    WB analysis using 16628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using human COPS7A Polyclonal antibody (16628-1-AP)

    IP experiment of HeLa using 16628-1-AP

    IP result of anti-COPS7A (IP:16628-1-AP, 4ug; Detection:16628-1-AP 1:5000) with HeLa cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using COPS7A Polyclonal antibody (16628-1-AP)

    IHC staining of mouse brain using 16628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16628-1-AP (COPS7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using COPS7A Polyclonal antibody (16628-1-AP)

    IF Staining of MCF-7 using 16628-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using COPS7A antibody (16628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inRAW 264.7 cells, mouse brain tissue, PC-12 cells, HeLa cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16628-1-AP targets COPS7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPS7A fusion protein Ag9958 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis)
Calculated molecular weight 275 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC011789
Gene symbol COPS7A
Gene ID (NCBI) 50813
RRIDAB_2081787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPS7A is component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. COPS7A was consistently expressed in oligodendrocyte lineage cells, and was down-regulated during the peak period of myelination. COPS7A associated physically with Olig1, and it might thus serve as a novel negative regulator in oligodendrocytes differentiation.

Protocols

Product Specific Protocols
WB protocol for COPS7A antibody 16628-1-APDownload protocol
IHC protocol for COPS7A antibody 16628-1-APDownload protocol
IF protocol for COPS7A antibody 16628-1-APDownload protocol
IP protocol for COPS7A antibody 16628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols