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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPZ1 Polyclonal antibody (20440-1-AP)

    WB analysis using 20440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using COPZ1 Polyclonal antibody (20440-1-AP)

    WB analysis of Jurkat using 20440-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using COPZ1 Polyclonal antibody (20440-1-AP)

    WB analysis of K-562 using 20440-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using COPZ1 Polyclonal antibody (20440-1-AP)

    WB analysis of COS-7 using 20440-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using COPZ1 Polyclonal antibody (20440-1-AP)

    IF Staining of HEK-293 using 20440-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 20440-1-AP (COPZ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

COPZ1 Polyclonal antibody

COPZ1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20440-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

HSPC181, COPZ, Coatomer subunit zeta-1, Coatomer subunit zeta 1, CGI-120

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOS-7 cells, K-562 cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20440-1-AP targets COPZ1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPZ1 fusion protein Ag14234 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit zeta 1
Calculated molecular weight 177 aa, 20 kDa
Observed molecular weight 17-20 kDa
GenBank accession numberBC002849
Gene Symbol COPZ1
Gene ID (NCBI) 22818
RRIDAB_10733095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61923
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPZ1 antibody 20440-1-APDownload protocol
IF protocol for COPZ1 antibody 20440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

MedComm (2020)

Chemoproteomics enables identification of coatomer subunit zeta-1 targeted by a small molecule for enterovirus A71 inhibition

Authors - Xiaoyong Li
View Article
mouseWB

Biochim Biophys Acta Gen Subj

COPZ1 regulates ferroptosis through NCOA4-mediated ferritinophagy in lung adenocarcinoma

Authors - Anbang Wu
  • KD Validated
View Article
humanWB

Genes (Basel)

A Comprehensive Pan-Cancer Analysis of the Regulation and Prognostic Effect of Coat Complex Subunit Zeta 1

Authors - Ye Hong
  • KD Validated
View Article