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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COQ10B Polyclonal antibody (17269-1-AP)

    WB analysis using 17269-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17269-1-AP (COQ10B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using COQ10B Polyclonal antibody (17269-1-AP)

    IF Staining of A431 using 17269-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COQ10B antibody (17269-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

COQ10B Polyclonal antibody

COQ10B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17269-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Coenzyme Q-binding protein COQ10 homolog B, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17269-1-AP targets COQ10B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ10B fusion protein Ag11024 相同性解析による交差性が予測される生物種
Full Name coenzyme Q10 homolog B (S. cerevisiae)
Calculated molecular weight 238 aa, 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC022453
Gene Symbol COQ10B
Gene ID (NCBI) 80219
RRIDAB_3669265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COQ10B (coenzyme Q10, homolog B) is an anti-apoptosis enzyme stored in the mitochondria of cells and belongs to the COQ10 family. COQ10B is an important biological cofactor, which can increase the concentration of mitochondria in the brain and play a neuroprotective role (PMID: 35613207). COQ10B is an isoform of COQ10 and has been detected in chronic stress-associated intestinal barrier dysfunction, acute pelvic inflammatory illness, moyamoya disease, and pelvic inflammatory disease (PMID: 35911165). The calculated molecular mass of COQ10B is 27 kDa.

Protocols

Product Specific Protocols
WB protocol for COQ10B antibody 17269-1-APDownload protocol
IF protocol for COQ10B antibody 17269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols