COQ5 Polyclonal antibody

Name: COQ5 Polyclonal antibody
Brand: Proteintech
SKU: 17453-1-AP

COQ5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17453-1-AP

Synonyms

COQ5

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of mouse heart using 17453-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17453-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 17453-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17453-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 17453-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 17453-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse heart tissue, mouse brain tissue, mouse liver tissue
Positive IHC detected in	human heart tissue, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IF	See 1 publications below

Product Information

17453-1-AP targets COQ5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	COQ5 fusion protein Ag10203 相同性解析による交差性が予測される生物種
Full Name	coenzyme Q5 homolog, methyltransferase (S. cerevisiae)
Calculated molecular weight	327 aa, 37 kDa
Observed molecular weight	34.7 kDa
GenBank accession number	BC107874
Gene symbol	COQ5
Gene ID (NCBI)	84274
RRID	AB_10547974
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for COQ5 antibody 17453-1-AP	Download protocol
IHC protocol for COQ5 antibody 17453-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	J Exp Med Implication of folate deficiency in CYP2U1 loss of function. Authors - Claire Pujol View Article
mouse	WB	EMBO Mol Med β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice. Authors - Agustín Hidalgo-Gutiérrez View Article
mouse	WB	Redox Biol The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency. Authors - Pilar González-García View Article
human	WB	J Am Soc Nephrol ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment. Authors - Eugen Widmeier View Article
mouse	WB	EMBO Mol Med The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene. Authors - Marta Luna-Sánchez View Article
mouse	WB	Sci Rep Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis. Authors - María Rodríguez-Hidalgo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COQ5 Polyclonal antibody

COQ5 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of mouse heart using 17453-1-AP

WB analysis of mouse liver using 17453-1-AP

WB analysis of mouse heart using 17453-1-AP

WB analysis of mouse brain using 17453-1-AP

IHC staining of human heart using 17453-1-AP

IHC staining of human heart using 17453-1-AP

IHC staining of human kidney using 17453-1-AP

IHC staining of human kidney using 17453-1-AP

IHC staining of human placenta using 17453-1-AP

IHC staining of human placenta using 17453-1-AP

IHC staining of human testis using 17453-1-AP

IHC staining of human testis using 17453-1-AP

IHC staining of human skin using 17453-1-AP

IHC staining of human skin using 17453-1-AP

IHC staining of human brain using 17453-1-AP

IHC staining of human brain using 17453-1-AP

IHC staining of human liver using 17453-1-AP

IHC staining of human liver using 17453-1-AP

IHC staining of human spleen using 17453-1-AP

IHC staining of human spleen using 17453-1-AP

IHC staining of human lung using 17453-1-AP

IHC staining of human lung using 17453-1-AP

IHC staining of human ovary using 17453-1-AP

IHC staining of human ovary using 17453-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Implication of folate deficiency in CYP2U1 loss of function.

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.

ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment.

The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene.

Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis.