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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using COQ6 Polyclonal antibody (12481-1-AP)

    WB analysis of mouse heart using 12481-1-AP (same clone as 12481-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12481-1-AP (COQ6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COQ6 Polyclonal antibody (12481-1-AP)

    WB analysis using 12481-1-AP (same clone as 12481-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12481-1-AP (COQ6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using COQ6 Polyclonal antibody (12481-1-AP)

    IHC staining of mouse ovary using 12481-1-AP (same clone as 12481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12481-1-AP (COQ6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using COQ6 Polyclonal antibody (12481-1-AP)

    IHC staining of mouse ovary using 12481-1-AP (same clone as 12481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12481-1-AP (COQ6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COQ6 Polyclonal antibody (12481-1-AP)

    IHC staining of human heart using 12481-1-AP (same clone as 12481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12481-1-AP (COQ6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

COQ6 Polyclonal antibody, PBS Only

COQ6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12481-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12481-1-PBS targets COQ6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ6 fusion protein Ag3164 相同性解析による交差性が予測される生物種
Full Name coenzyme Q6 homolog, monooxygenase (S. cerevisiae)
Calculated molecular weight 468 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC014181
Gene Symbol COQ6
Gene ID (NCBI) 51004
RRIDAB_10644143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Z9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COQ6(Coenzyme Q10 monooxygenase 6) is also named CGI-10 and belongs to the ubiH/COQ6 family. It is an evolutionarily conserved flavin-dependent monooxygenase required for the biosynthesis of coenzyme Q10 or ubiquinone. It has 3 isoforms with a molecular mass of 51 kDa, 43 kDa and 49 kDa. In the inner ear, Coq6 is expressed in the spiral ganglion, stria vascularis cells, and spiral ligament(PMID:21540551 ). Defects in COQ6 are the cause of coenzyme Q10 deficiency, primary, type 6 (COQ10D6).