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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CORIN Recombinant antibody

CORIN Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240339A10

Cat no : 83566-1-RR

Synonyms

TMPRSS10, serine peptidase, CRN, 240339A10

Validation Data Gallery

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  • Western Blot (WB) analysis of rat heart tissue using CORIN Recombinant antibody (83566-1-RR)

    WB analysis of rat heart using 83566-1-RR

    rat heart tissue were subjected to SDS PAGE followed by western blot with 83566-1-RR (CORIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CORIN Recombinant antibody (83566-1-RR)

    IHC staining of mouse heart using 83566-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83566-1-RR (CORIN antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CORIN Recombinant antibody (83566-1-RR)

    IHC staining of mouse heart using 83566-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83566-1-RR (CORIN antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83566-1-RR

    Affinity and Kinetic Characterization of 83566-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83566-1-RR against Human CORIN were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inrat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83566-1-RR is CORIN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CORIN fusion protein Ag15898 相同性解析による交差性が予測される生物種
Full Name corin, serine peptidase
Calculated molecular weight 1042 aa, 117 kDa
Observed molecular weight180 kDa
GenBank accession numberBC110451
Gene symbol CORIN
Gene ID (NCBI) 10699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CORIN is a transmembrane type II serine protease expressed in cardiomyocytes that converts pro-atrial natriuretic peptide into atrial natriuretic peptide, a cardiac hormone that regulates blood pressure. CORIN can be shed into the circulation through autocleavage and metalloproteinase-mediated hydrolysis processes of the membrane form of the protein. Three different soluble fragments derived from corin molecules, with molecular masses of ~180, ~160 and ~100 kDa were described in literature (PMID: 21288900, 29387201).

Protocols

Product Specific Protocols
WB protocol for CORIN antibody 83566-1-RRDownload protocol
IHC protocol for CORIN antibody 83566-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols