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  • Featured Product
  • KD/KO Validated

CORO2A Polyclonal antibody

CORO2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10119-1-AP

Synonyms

CLIPINB, CORO2A, Coronin 2A, CRN5, IR10, WD repeat containing protein 2, WDR2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CORO2A Polyclonal antibody (10119-1-AP)

    WB analysis using 10119-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10119-1-AP (CORO2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CORO2A Polyclonal antibody (10119-1-AP)

    WB analysis of Jurkat using 10119-1-AP

    WB result of CORO2A antibody (10119-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CORO2A transfected Jurkat cells.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CORO2A Polyclonal antibody (10119-1-AP)

    IHC staining of human skin cancer using 10119-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10119-1-AP (CORO2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CORO2A Polyclonal antibody (10119-1-AP)

    IHC staining of human skin cancer using 10119-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10119-1-AP (CORO2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CORO2A Polyclonal antibody (10119-1-AP)

    IHC staining of mouse small intestine using 10119-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 10119-1-AP (CORO2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CORO2A Polyclonal antibody (10119-1-AP)

    IHC staining of mouse small intestine using 10119-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 10119-1-AP (CORO2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman skin cancer tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10119-1-AP targets CORO2A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO2A fusion protein Ag0164 相同性解析による交差性が予測される生物種
Full Name coronin, actin binding protein, 2A
Calculated molecular weight 60 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000010
Gene symbol CORO2A
Gene ID (NCBI) 7464
RRIDAB_2877724
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CORO2A (Coronin 2A), also named as CLIPINB, is a member of the 'short' coronin subfamily containing a single WD40-repeat domain. It has been shown that the expression of CORO2A is up-regulated in human colon cancer. CORO2A can interact with MAPK14 and PRMT5. The calculated MW of CORO2A is 60 kDa, while 10119-1-AP antibody detects 48 kDa protein which is similar to the paper published. (PMID: 26373535)

Protocols

Product Specific Protocols
WB protocol for CORO2A antibody 10119-1-APDownload protocol
IHC protocol for CORO2A antibody 10119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols