☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

COTL1 Polyclonal antibody

COTL1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10781-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

CLP, Coactosin like protein, COTL1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10781-1-AP targets COTL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COTL1 fusion protein Ag1230 相同性解析による交差性が予測される生物種
Full Name coactosin-like 1 (Dictyostelium)
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC010884
Gene Symbol COTL1
Gene ID (NCBI) 23406
RRIDAB_2084785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14019
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Protocols

Product Specific Protocols
WB protocol for COTL1 antibody 10781-1-APDownload protocol
IHC protocol for COTL1 antibody 10781-1-APDownload protocol
IP protocol for COTL1 antibody 10781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Proteome Res

Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.

Authors - Jeong Hye-Cheol HC
humanWB

Oncotarget

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.

Authors - Shanqi Guo
humanIHC

J Inflamm Res

Coactosin-Like Protein in Breast Carcinoma: Friend or Foe?

Authors - Bei Wang
humanWB

PLoS One

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

Authors - Somalinga Balajee R BR
  • KD Validated
humanWB

Exp Lung Res

Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells.

Authors - Claudia D'Anna
humanWB

J Investig Med

Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.

Authors - Sun Wenjing W
Loading...