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COTL1 Monoclonal antibody
COTL1 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
4A3D9
Cat no : 60237-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, fetal human brain tissue, human peripheral blood platelets, HeLa cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
60237-1-Ig targets COTL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | COTL1 fusion protein Ag1230 相同性解析による交差性が予測される生物種 |
Full Name | coactosin-like 1 (Dictyostelium) |
Calculated molecular weight | 16 kDa |
Observed molecular weight | 14-16 kDa |
GenBank accession number | BC010884 |
Gene symbol | COTL1 |
Gene ID (NCBI) | 23406 |
RRID | AB_2881360 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.
Protocols
Product Specific Protocols | |
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WB protocol for COTL1 antibody 60237-1-Ig | Download protocol |
IHC protocol for COTL1 antibody 60237-1-Ig | Download protocol |
IF protocol for COTL1 antibody 60237-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |