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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COX18 Polyclonal antibody

COX18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18071-1-AP

Synonyms

OXA1L2, Cytochrome c oxidase assembly protein COX18, mitochondrial, COX18Hs

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using COX18 Polyclonal antibody (18071-1-AP)

    WB analysis of mouse liver using 18071-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18071-1-AP (COX18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using COX18 Polyclonal antibody (18071-1-AP)

    WB analysis of NIH/3T3 using 18071-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18071-1-AP (COX18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using COX18 Polyclonal antibody (18071-1-AP)

    WB analysis of HeLa using 18071-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18071-1-AP (COX18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using COX18 Polyclonal antibody (18071-1-AP)

    IHC staining of human ovary cancer using 18071-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18071-1-AP (COX18 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COX18 Polyclonal antibody (18071-1-AP)

    IF Staining of HepG2 using 18071-1-AP

    Immunofluorescent analysis of HepG2 cells, using COX18 antibody 18071-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18071-1-AP targets COX18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX18 fusion protein Ag12709 相同性解析による交差性が予測される生物種
Full Name COX18 cytochrome c oxidase assembly homolog (S. cerevisiae)
Calculated molecular weight 333 aa, 37 kDa
Observed molecular weight 37-40 kDa, 28-30 kDa
GenBank accession numberBC101684
Gene symbol COX18
Gene ID (NCBI) 285521
RRIDAB_10638443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COX18 antibody 18071-1-APDownload protocol
IHC protocol for COX18 antibody 18071-1-APDownload protocol
IF protocol for COX18 antibody 18071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis.

Authors - Isotta Lorenzi
View Article