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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COX18 Recombinant antibody

COX18 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241359G7

Cat no : 84377-3-RR

Synonyms

OXA1L2, Cytochrome c oxidase assembly protein COX18, mitochondrial, COX18Hs, 241359G7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COX18 Recombinant antibody (84377-3-RR)

    WB analysis using 84377-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84377-3-RR (COX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84377-3-RR

    Affinity and Kinetic Characterization of 84377-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84377-3-RR against Human COX18 were performed. The affinity constant is 11.0 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84377-3-RR targets COX18 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COX18 fusion protein Ag12709 相同性解析による交差性が予測される生物種
Full Name COX18 cytochrome c oxidase assembly homolog (S. cerevisiae)
Calculated molecular weight 333 aa, 37 kDa
Observed molecular weight 37-40 kDa, 28-30 kDa
GenBank accession numberBC101684
Gene symbol COX18
Gene ID (NCBI) 285521
RRIDAB_3671914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COX18 antibody 84377-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols