Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11242-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11242-1-AP
WB result of COXIV antibody (11242-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells.
WB analysis using 11242-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 11242-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 11242-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 11242-1-AP
IP result of anti-COXIV (IP:11242-1-AP, 3ug; Detection:11242-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3500ug.
IHC staining of human prostate cancer using 11242-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11242-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 11242-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 11242-1-AP (COXIV antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 11242-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 11242-1-AP (COXIV antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas cancer using 11242-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11242-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11242-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (11242-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

COXIV Polyclonal antibody

COXIV Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11242-1-AP

Publications(498)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

COX4I1, COX IV-1, COX4, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (498)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, rat liver tissue, rat brain tissue, MCF-7 cells, NIH/3T3 cells, Jurkat cells, mouse skeletal muscle tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human prostate cancer tissue, human pancreas cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:20000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 449 publications below
IHC	See 9 publications below
IF	See 54 publications below
IP	See 3 publications below
ChIP	See 1 publications below

Product Information

11242-1-AP targets COXIV in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, bovine, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1640 Product name: Recombinant human COXIV protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-169 aa of BC021236 Sequence: MLATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEWKTVVGGAMFFIGFTALVIMWQKHYVYGPLPQSFDKEWVAKQTKRMLDMKVNPIQGLASKWDYEKNEWKK 相同性解析による交差性が予測される生物種
Full Name	cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight	19.6 kDa
Observed molecular weight	17-18 kDa
GenBank accession number	BC021236
Gene Symbol	COX IV
Gene ID (NCBI)	1327
RRID	AB_2085278
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13073
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.

Protocols

Product Specific Protocols
FC protocol for COXIV antibody 11242-1-AP	Download protocol
IF protocol for COXIV antibody 11242-1-AP	Download protocol
IHC protocol for COXIV antibody 11242-1-AP	Download protocol
IP protocol for COXIV antibody 11242-1-AP	Download protocol
WB protocol for COXIV antibody 11242-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression Authors - Shiqiao Qiu View Article
	WB	Cell Res Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses Authors - Qihang Zhao View Article
mouse,human	WB,IF	Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. Authors - Fangzhou Lou View Article
mouse	WB	Nat Metab Time of exercise differentially impacts bone growth in mice Authors - Shaoling Yu View Article
human	WB	Cell Metab Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway. Authors - Kiran Kurmi View Article
mouse	WB	Nat Cell Biol AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1. Authors - Meng Shi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11242-1-AP

WB analysis of HepG2 using 11242-1-AP

WB analysis using 11242-1-AP

WB analysis of rat brain using 11242-1-AP

WB analysis of rat liver using 11242-1-AP

IP experiment of mouse skeletal muscle using 11242-1-AP

IHC staining of human prostate cancer using 11242-1-AP

IHC staining of human prostate cancer using 11242-1-AP

IHC staining of human heart using 11242-1-AP

IHC staining of human heart using 11242-1-AP

IHC staining of human pancreas cancer using 11242-1-AP

IHC staining of human pancreas cancer using 11242-1-AP

IF Staining of HepG2 using 11242-1-AP

COXIV Polyclonal antibody

COXIV Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Time of exercise differentially impacts bone growth in mice

Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.