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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis using 11463-1-AP (same clone as 11463-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis of human brain using 11463-1-AP (same clone as 11463-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis of MCF-7 using 11463-1-AP (same clone as 11463-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis of HepG2 using 11463-1-AP (same clone as 11463-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis of A375 using 11463-1-AP (same clone as 11463-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis of MCF-7 using 11463-1-AP (same clone as 11463-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis of human lung using 11463-1-AP (same clone as 11463-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis of human brain using 11463-1-AP (same clone as 11463-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using COX4I2 Polyclonal antibody (11463-1-AP)

    WB analysis of human heart using 11463-1-AP (same clone as 11463-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment using COX4I2 Polyclonal antibody (11463-1-AP)

    IP experiment using 11463-1-AP (same clone as 11463-1-PBS)

    IP result of anti-COX4I2 (11463-1-AP for IP and Detection) with HepG2 cell lysate. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using COX4I2 Polyclonal antibody (11463-1-AP)

    IP experiment of HepG2 using 11463-1-AP (same clone as 11463-1-PBS)

    IP result of anti-COX4I2 (IP:11463-1-AP, 3ug; Detection:11463-1-AP 1:1000) with HepG2 cells lysate 8000ug. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using COX4I2 Polyclonal antibody (11463-1-AP)

    IHC staining of human ovary tumor using 11463-1-AP (same clone as 11463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11463-1-AP (COX4I2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COX4I2 Polyclonal antibody (11463-1-AP)

    IF Staining of HeLa using 11463-1-AP (same clone as 11463-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX4I2 antibody (11463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COX4I2 Polyclonal antibody (11463-1-AP)

    IF Staining of HepG2 using 11463-1-AP (same clone as 11463-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX4I2 antibody (11463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

COX4I2 Polyclonal antibody, PBS Only

COX4I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11463-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

COX4L2, COX4B, COX4 2, COX4, COX IV-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11463-1-PBS targets COX4I2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX4I2 fusion protein Ag1987 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit IV isoform 2 (lung)
Calculated molecular weight 171 aa, 20 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC057779
Gene Symbol COX4I2
Gene ID (NCBI) 84701
RRIDAB_2085287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KJ9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.