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  • Featured Product
  • KD/KO Validated

COX5B Polyclonal antibody, PBS Only

COX5B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11418-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using COX5B Polyclonal antibody (11418-2-AP)

    WB analysis of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

    WB result of COX5B antibody (11418-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX5B transfected HeLa cells. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COX5B Polyclonal antibody (11418-2-AP)

    WB analysis using 11418-2-AP (same clone as 11418-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11418-2-AP (COX5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using COX5B Polyclonal antibody (11418-2-AP)

    IHC staining of human liver cancer using 11418-2-AP (same clone as 11418-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COX5B Polyclonal antibody (11418-2-AP)

    IHC staining of human breast cancer using 11418-2-AP (same clone as 11418-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COX5B Polyclonal antibody (11418-2-AP)

    IF Staining of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11418-2-AP (COX5B antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11418-2-PBS targets COX5B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX5B fusion protein Ag1986 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit Vb
Calculated molecular weight 129 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006229
Gene symbol COX5B
Gene ID (NCBI) 1329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)