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  • Featured Product
  • KD/KO Validated

COX5B Polyclonal antibody, PBS Only

COX5B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11418-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using COX5B Polyclonal antibody (11418-2-AP)

    WB analysis of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

    WB result of COX5B antibody (11418-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX5B transfected HeLa cells. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COX5B Polyclonal antibody (11418-2-AP)

    WB analysis using 11418-2-AP (same clone as 11418-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11418-2-AP (COX5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using COX5B Polyclonal antibody (11418-2-AP)

    IHC staining of human liver cancer using 11418-2-AP (same clone as 11418-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COX5B Polyclonal antibody (11418-2-AP)

    IHC staining of human breast cancer using 11418-2-AP (same clone as 11418-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COX5B Polyclonal antibody (11418-2-AP)

    IF Staining of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11418-2-AP (COX5B antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11418-2-PBS targets COX5B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX5B fusion protein Ag1986 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit Vb
Calculated molecular weight 129 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006229
Gene symbol COX5B
Gene ID (NCBI) 1329
RRIDAB_2245376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)