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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COX6B2 Polyclonal antibody

COX6B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11437-1-AP

Synonyms

Cancer/testis antigen 59, COX VIb 2, COX6B2, COXVIB2, CT59

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using COX6B2 Polyclonal antibody (11437-1-AP)

    WB analysis using 11437-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11437-1-AP (COX6B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using COX6B2 Polyclonal antibody (11437-1-AP)

    IHC staining of mouse testis using 11437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using COX6B2 Polyclonal antibody (11437-1-AP)

    IHC staining of mouse testis using 11437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11437-1-AP targets COX6B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX6B2 fusion protein Ag1983 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit VIb polypeptide 2 (testis)
Calculated molecular weight 88 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC064548
Gene symbol COX6B2
Gene ID (NCBI) 125965
RRIDAB_10859092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX6B2(Cytochrome c oxidase subunit 6B2) encodes the testis-specific cytochrome C oxidase subunit VIb. It has been shown to be expressed in a subset of lung and breast tumors at a level >1% of the testicular level of expression(PMID: 24491090). It belongs to the cytochrome c oxidase subunit 6B family.

Protocols

Product Specific Protocols
WB protocol for COX6B2 antibody 11437-1-APDownload protocol
IHC protocol for COX6B2 antibody 11437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

PMID: 20870728

Authors - Oliva Claudia R CR
View Article