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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COXIV Monoclonal antibody, PBS Only (Capture)

COXIV Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7B2

Cat no : 66110-1-PBS

Synonyms

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1, 2A7B2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COXIV Monoclonal antibody (66110-1-Ig)

    WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COXIV Monoclonal antibody (66110-1-Ig)

    WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using COXIV Monoclonal antibody (66110-1-Ig)

    WB analysis of HeLa using 66110-1-Ig (same clone as 66110-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Monoclonal antibody (66110-1-Ig)

    IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50551-3

    Cytometric bead array standard curve of MP50551-3

    Cytometric bead array standard curve of MP50551-3, COXIV Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66110-1-PBS. Detection antibody: 60251-4-PBS. Standard:Ag1640. Range: 1.563-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66110-1-PBS targets COXIV as part of a matched antibody pair:

MP50551-3: 66110-1-PBS capture and 60251-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COXIV fusion protein Ag20551 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight19.6 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC021236
Gene symbol COX IV
Gene ID (NCBI) 1327
RRIDAB_2881509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.