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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using COXIV Recombinant antibody (82916-1-RR)

    WB analysis of HepG2 using 82916-1-RR (same clone as 82916-1-PBS)

    WB result of COXIV antibody (82916-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COXIV Recombinant antibody (82916-1-RR)

    WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COXIV Recombinant antibody (82916-1-RR)

    WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COXIV Recombinant antibody (82916-1-RR)

    WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using COXIV Recombinant antibody (82916-1-RR)

    IHC staining of human stomach using 82916-1-RR (same clone as 82916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using COXIV Recombinant antibody (82916-1-RR)

    IHC staining of human colon using 82916-1-RR (same clone as 82916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using COXIV Recombinant antibody (82916-1-RR)

    IHC staining of human ovarian cancer using 82916-1-RR (same clone as 82916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82916-1-RR (COXIV antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COXIV Recombinant antibody (82916-1-RR)

    IF Staining of HepG2 using 82916-1-RR (same clone as 82916-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (82916-1-RR, Clone: 3K20 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using COXIV Recombinant antibody (82916-1-RR)

    FC experiment of HeLa using 82916-1-RR (same clone as 82916-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug COXIV Recombinant antibody (82916-1-RR, Clone:3K20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82916-1-RR

    Affinity and Kinetic Characterization of 82916-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82916-1-RR against Human COXIV were performed. The affinity constant is 9.41 nM.

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  • Featured Product
  • KD/KO Validated

COXIV Recombinant antibody, PBS Only

COXIV Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82916-1-PBS
Clone No.3K20

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1, 3K20

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82916-1-PBS targets COXIV in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COXIV fusion protein Ag1640 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight19.6 kDa
Observed molecular weight17-18 kDa
GenBank accession numberBC021236
Gene Symbol COX IV
Gene ID (NCBI) 1327
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13073
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues.