CoraLite® Plus 488-conjugated COXIV Monoclonal antibody
COXIV Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C7D2
Cat no : CL488-60251
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
CL488-60251 targets COXIV in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | COXIV fusion protein Ag20551 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome c oxidase subunit IV isoform 1 |
| Calculated molecular weight | 19.6 kDa |
| Observed molecular weight | 17-18 kDa |
| GenBank accession number | BC021236 |
| Gene symbol | COX IV |
| Gene ID (NCBI) | 1327 |
| RRID | AB_2883126 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 COXIV antibody CL488-60251 | Download protocol |
| FC protocol for CL Plus 488 COXIV antibody CL488-60251 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis | ||
Life Sci Short-term but not long-term high fat diet feeding protects against pressure overload-induced heart failure through activation of mitophagy. |
