Validation Data Gallery
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Tested Applications
| Positive WB detected in | human blood tissue, mouse brain tissue, human plasma tissue |
| Positive IHC detected in | human tonsillitis tissue, human colon tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
21131-1-AP targets Ceruloplasmin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Ceruloplasmin fusion protein Ag15535 相同性解析による交差性が予測される生物種 |
| Full Name | ceruloplasmin (ferroxidase) |
| Calculated molecular weight | 1065 aa, 122 kDa |
| Observed molecular weight | 130-140 kDa, 85 kDa |
| GenBank accession number | BC146663 |
| Gene Symbol | Ceruloplasmin |
| Gene ID (NCBI) | 1356 |
| RRID | AB_2878815 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00450 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Ceruloplasmin antibody 21131-1-AP | Download protocol |
| IHC protocol for Ceruloplasmin antibody 21131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer
| ||
J Agric Food Chem Drp1 Aggravates Copper Nanoparticle-Induced ER-Phagy by Disturbing Mitochondria-Associated Membranes in Chicken Hepatocytes | ||
J Neuroinflammation Aberrant copper metabolism and hepatic inflammation cause neurological manifestations in a mouse model of Wilson's disease | ||
Cells Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis | ||
J Clin Pharmacol Expression of ceruloplasmin in the peripheral blood of patients with drug-resistant epilepsy | ||
Cell Death Dis The divergent effects of astrocyte ceruloplasmin on learning and memory function in young and old mice |