• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CP110 Recombinant antibody

CP110 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242016E10

Cat no : 80965-5-RR

Synonyms

CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CP110 Recombinant antibody (80965-5-RR)

    WB analysis using 80965-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80965-5-RR (CP110 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CP110 Recombinant antibody (80965-5-RR)

    IHC staining of mouse testis using 80965-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80965-5-RR (CP110 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CP110 Recombinant antibody (80965-5-RR)

    IF Staining of HepG2 using 80965-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CP110 antibody (80965-5-RR, Clone: 242016E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80965-5-RR

    Affinity and Kinetic Characterization of 80965-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80965-5-RR against Human CP110 were performed. The affinity constant is below 1 pM.

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, MOLT-4 cells, HCT 116 cells, HeLa cells, K-562 cells, U2OS cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80965-5-RR targets CP110 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CP110 fusion protein Ag3489 相同性解析による交差性が予測される生物種
Full Name CP110 protein
Calculated molecular weight 991 aa, 111 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC036654
Gene symbol CP110
Gene ID (NCBI) 9738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

Protocols

Product Specific Protocols
WB protocol for CP110 antibody 80965-5-RRDownload protocol
IHC protocol for CP110 antibody 80965-5-RRDownload protocol
IF protocol for CP110 antibody 80965-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols