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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPA1 Monoclonal antibody (68826-1-Ig)

    WB analysis using 68826-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68826-1-Ig (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CPA1 Monoclonal antibody (68826-1-Ig)

    IF Staining of mouse pancreas using 68826-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPA1 antibody (68826-1-Ig, Clone: 2H2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CPA1 Monoclonal antibody

CPA1 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68826-1-Ig
Clone No.2H2E8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, ELISA

Synonyms

Carboxypeptidase A1, EC:3.4.17.1, CPA, 2H2E8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig pancreas tissue, rat pancreas tissue, mouse pancreas tissue
Positive IF-P detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68826-1-Ig targets CPA1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CPA1 fusion protein Ag8579 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase A1 (pancreatic)
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC005279
Gene Symbol Carboxypeptidase A1
Gene ID (NCBI) 1357
RRIDAB_3670437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.

Protocols

Product Specific Protocols
WB protocol for CPA1 antibody 68826-1-IgDownload protocol
IF protocol for CPA1 antibody 68826-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols