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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Carboxypeptidase A3 Polyclonal antibody

Carboxypeptidase A3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16236-1-AP

Synonyms

CPA3, MC-CPA, MC CPA, Mast cell carboxypeptidase A, EC:3.4.17.1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    WB analysis of mouse pancreas using 16236-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    WB analysis of mouse pancreas using 16236-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    IHC staining of human tonsillitis using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    IHC staining of human small intestine using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    IHC staining of human small intestine using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    IHC staining of human tonsillitis using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    IHC staining of human tonsillitis using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    IHC staining of human tonsillitis using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    IHC staining of human pancreas using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Carboxypeptidase A3 Polyclonal antibody (16236-1-AP)

    IHC staining of human pancreas using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman tonsillitis tissue, human pancreas tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16236-1-AP targets Carboxypeptidase A3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase A3 fusion protein Ag8984 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase A3 (mast cell)
Calculated molecular weight 417 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC012613
Gene symbol Carboxypeptidase A3
Gene ID (NCBI) 1359
RRIDAB_2085856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Carboxypeptidase A3 antibody 16236-1-APDownload protocol
IHC protocol for Carboxypeptidase A3 antibody 16236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Pathol

Rare Pulmonary Connective Tissue Type Mast Cells Regulate Lung Endothelial Cell Angiogenesis.

Authors - Yue Ren
View Article