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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Carboxypeptidase B2 Polyclonal antibody

Carboxypeptidase B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10672-1-AP

Synonyms

Carboxypeptidase B2, carboxypeptidase B2 (plasma), Carboxypeptidase U, CPB2, CPU, PCPB, Plasma carboxypeptidase B, TAFI

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using Carboxypeptidase B2 Polyclonal antibody (10672-1-AP)

    WB analysis of mouse liver using 10672-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Carboxypeptidase B2 Polyclonal antibody (10672-1-AP)

    IHC staining of human liver cancer using 10672-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Carboxypeptidase B2 Polyclonal antibody (10672-1-AP)

    IHC staining of human colon cancer using 10672-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Carboxypeptidase B2 Polyclonal antibody (10672-1-AP)

    IHC staining of human liver using 10672-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue, human liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10672-1-AP targets Carboxypeptidase B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase B2 fusion protein Ag1110 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase B2 (plasma)
Calculated molecular weight 48 kDa
Observed molecular weight53 kDa
GenBank accession numberBC007057
Gene symbol Carboxypeptidase B2
Gene ID (NCBI) 1361
RRIDAB_2086000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPB2(Carboxypeptidase B2) belongs to the peptidase M14 family. The CPB2 gene encoding TAFI is a candidate gene for blood pressure. Circulating CPB2 and platelets may potentially contribute to regulating the bioactivity of leukocyte chemoattractant chemerin (RARRES2), and further extend the molecular link between blood coagulation/fibrinolysis and CMKLR1-mediated immune responses. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Carboxypeptidase B2 antibody 10672-1-APDownload protocol
IHC protocol for Carboxypeptidase B2 antibody 10672-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FEMS Immunol Med Microbiol

Secreted Bacillus anthracis proteases target the host fibrinolytic system.

Authors - Chung Myung-Chul MC
View Article
mouseWB

Neurochem Res

Serum Proteomics Identified TAFI as a Potential Molecule Facilitating the Migration of Peripheral Monocytes to Damaged White Matter During Chronic Cerebral Hypoperfusion

Authors - Yuhan Wang
View Article