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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPEB1 Polyclonal antibody (13274-1-AP)

    WB analysis using 13274-1-AP (same clone as 13274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13274-1-AP (CPEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CPEB1 Polyclonal antibody (13274-1-AP)

    IHC staining of human breast cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CPEB1 Polyclonal antibody (13274-1-AP)

    IHC staining of human breast cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPEB1 Polyclonal antibody (13274-1-AP)

    IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPEB1 Polyclonal antibody (13274-1-AP)

    IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPEB1 Polyclonal antibody (13274-1-AP)

    IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPEB1 Polyclonal antibody (13274-1-AP)

    IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CPEB1 Polyclonal antibody (13274-1-AP)

    IHC staining of human colon cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CPEB1 Polyclonal antibody (13274-1-AP)

    IHC staining of human colon cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CPEB1 Polyclonal antibody (13274-1-AP)

    IF Staining of HeLa using 13274-1-AP (same clone as 13274-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CPEB1 antibody (13274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CPEB1 Polyclonal antibody, PBS Only

CPEB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13274-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13274-1-PBS targets CPEB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPEB1 fusion protein Ag3994 相同性解析による交差性が予測される生物種
Full Name cytoplasmic polyadenylation element binding protein 1
Calculated molecular weight 486 aa, 54 kDa
Observed molecular weight 60-70kDa
GenBank accession numberBC035348
Gene Symbol CPEB1
Gene ID (NCBI) 64506
RRIDAB_2083771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZB8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling. This is a rabbit polyclonal antibody raised against residues near the C terminus of human CPEB1.