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CPLX4 Polyclonal antibody

CPLX4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21222-1-AP

Synonyms

complexin 4, Complexin IV, CPLX4, CPX IV, CPXIV

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using CPLX4 Polyclonal antibody (21222-1-AP)

    WB analysis of Y79 using 21222-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CPLX4 Polyclonal antibody (21222-1-AP)

    WB analysis of Y79 using 21222-1-AP

    WB result of CPLX4 antibody (21222-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX4 transfected Y79 cells.

  • Western Blot (WB) analysis of Y79 cells using CPLX4 Polyclonal antibody (21222-1-AP)

    WB analysis of Y79 using 21222-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using CPLX4 Polyclonal antibody (21222-1-AP)

    IP experiment of Y79 using 21222-1-AP

    IP result of anti-CPLX4 (IP:21222-1-AP, 3ug; Detection:21222-1-AP 1:1000) with Y79 cells lysate 960ug.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CPLX4 Polyclonal antibody (21222-1-AP)

    IHC staining of mouse eye using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CPLX4 Polyclonal antibody (21222-1-AP)

    IHC staining of mouse eye using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using CPLX4 Polyclonal antibody (21222-1-AP)

    IHC staining of human brain using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21222-1-AP (CPLX4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inY79 cells
Positive IP detected inY79 cells
Positive IHC detected inmouse eye tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21222-1-AP targets CPLX4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPLX4 fusion protein Ag15661 相同性解析による交差性が予測される生物種
Full Name complexin 4
Calculated molecular weight 160 aa, 18 kDa
Observed molecular weight 21-23 kDa
GenBank accession numberBC106887
Gene symbol CPLX4
Gene ID (NCBI) 339302
RRIDAB_10733348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CPLX4 antibody 21222-1-APDownload protocol
IHC protocol for CPLX4 antibody 21222-1-APDownload protocol
IP protocol for CPLX4 antibody 21222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols