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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Carboxypeptidase M Polyclonal antibody (16440-1-AP)

    WB analysis of HEK-293 using 16440-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Carboxypeptidase M Polyclonal antibody (16440-1-AP)

    WB analysis of HEK-293 using 16440-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using Carboxypeptidase M Polyclonal antibody (16440-1-AP)

    WB analysis of mouse liver using 16440-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Carboxypeptidase M Polyclonal antibody

Carboxypeptidase M Polyclonal Antibody for WB, ELISA
Cat No. 16440-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Carboxypeptidase M, CPM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16440-1-AP targets Carboxypeptidase M in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase M fusion protein Ag9761 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase M
Calculated molecular weight 443 aa, 51 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC022276
Gene Symbol Carboxypeptidase M
Gene ID (NCBI) 1368
RRIDAB_2084046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14384
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPM(Carboxypeptidase M), a plasma membrane-bound enzyme, is present in many human organs and differs from other carboxypeptidases that cleave basic C-terminal amino acids(PMID:2394713). CPM is structurally, catalytically and immunologically distinct from other carboxypeptidases, and expressed on cell membranes in a glycosyl-phosphatidylinositol-anchored form and it can be a novel marker and cellular player in lipid uptake and/or metabolism of MAs by promoting foam cell formation (PMID:22157720). This full length protein has a signal peptide with 17 amino acid, a propeptide with 20 amino acid and five glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for Carboxypeptidase M antibody 16440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols